Composite
Part:BBa_K2996507:Design
Designed by: Shifeng Jiang Group: iGEM19_SJTU-BioX-Shanghai (2019-10-03)
combination of sgRNA production module and target-DBD binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 199
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We put the sgRNA production module to the upstream of the lure-DBD binding site part in order to lower the burden of other plasmids.
Source
Synthesis